Anti-SARS-CoV-2 Spike Protein (Trimer) Human IgG ELISA Kit

Catalog #: KAV00109 Specific References (0) DATASHEET

Bioactivity for Anti-SARS-CoV-2 Spike Protein (Trimer) Human IgG ELISA Kit ?>

Applications: Used for the quantitative determination of Anti-SARS-CoV-2 Spike Protein (Trimer) Human IgG concentration in serum and plasma.

Sample type: Plasma, Serum

Sensitivity: 54.8 pg/mL

Range: 125 - 8,000 pg/mL

Overview

Catalog No.

KAV00109

Description

This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 Spike Protein (Trimer) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human Anti-SARS-CoV-2 Spike Protein (Trimer) IgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human Anti-SARS-CoV-2 Spike Protein (Trimer) IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Applications

Used for the quantitative determination of Anti-SARS-CoV-2 Spike Protein (Trimer) Human IgG concentration in serum and plasma.

Target

Spike

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

125 - 8,000 pg/mL

Sensitivity

54.8 pg/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/mL)	2802.1	561.9	146.7	3509.3	606.9	161.6
Standard deviation	104.5	26.5	12.8	301.1	50.9	18.2
CV (%)	3.7	4.7	8.7	8.6	8.4	11.2

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.

Note

For Research Use Only.

Data Image

Experiment Example

CALCULATION OF RESULTS
Average the duplicate readings for each standard and sample. Construct a standard curve by plotting the mean absorbance for each standard on the Y-axis against the concentration on the X-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the Anti-SARS-CoV-2 Spike Protein (Trimer) Human IgG concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Bioactivity

The levels of anti-myelin, neuroactive, and anti-SARS-CoV-2 antibodies in serum.
Data sourced from citation (PMID: 41465566)

References

Datasheet

Document Download

Anti-SARS-CoV-2 Spike Protein (Trimer) Human IgG ELISA Kit.pdf

$ 548

Product specifications

96 T 548

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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